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Image Search Results
Journal: Immunity
Article Title: Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche
doi: 10.1016/j.immuni.2019.08.017
Figure Lengend Snippet:
Article Snippet:
Techniques: Control, Recombinant, Blocking Assay, Irradiation, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Microarray, Software, Microscopy
Journal: The Tohoku journal of experimental medicine
Article Title: Bone Morphogenetic Proteins Inhibit Ciliogenesis of Ependymal Cells in Vitro.
doi: 10.1620/tjem.252.199
Figure Lengend Snippet: Fig. 3. Effect of bone morphogenetic proteins (BMPs) on cell differentiation. The number of multi-ciliated cells per total cells (A), the density of multi-ciliated cells (B), and the density of total cells (C) in different BMPs concentrations and medium with vehicle (control) are shown. The same control data is used for analyses of both BMP2 conditions and BMP4 conditions. n = 8 or 9. Data are mean ± SEM. “†” indicates a significant difference between day 0 and the other days. “*” indicates significant difference between days except day 0. *p < 0.05, **p < 0.01, ***, †††p < 0.0001.
Article Snippet: In the second experiment,
Techniques: Cell Differentiation, Control
Journal: The Tohoku journal of experimental medicine
Article Title: Bone Morphogenetic Proteins Inhibit Ciliogenesis of Ependymal Cells in Vitro.
doi: 10.1620/tjem.252.199
Figure Lengend Snippet: Fig. 5. Phosphorylation of Smad1/5/8 proteins by BMP treatment in the primary ependymal cells. A. Signals of phosphorylated Smad1/5/8 (pSmad1/5/8) were detected in BMP2 or BMP4-treated cells (BMP2 or BMP4) but not in the control cells (Control). GAPDH was used as an internal control. B. The signal of non-phosphorylated Smad5 was similar in each condition.
Article Snippet: In the second experiment,
Techniques: Phospho-proteomics, Control
Journal: The Tohoku journal of experimental medicine
Article Title: Bone Morphogenetic Proteins Inhibit Ciliogenesis of Ependymal Cells in Vitro.
doi: 10.1620/tjem.252.199
Figure Lengend Snippet: Fig. 7. FoxJ1 and nestin expressions in the cultured cells. A. Representative image of cells cultured in medium without BMPs (control condition) for 14 days. A cilia marker, β-Tubulin IV, is stained in green, and FoxJ1, a nuclear marker for ciliated cells is stained in magenta. The nuclei are counterstained with DAPI in blue. Arrows indicate FoxJ1-positive cells without multiple cilia, and arrowheads indi- cate FoxJ1-positive cells with multiple cilia. Scale bar: 20 µm. B. The number of FoxJ1-positive cilia-positive cells (FoxJ1(+), Cilia(+)), FoxJ1-positive cilia-negative cells (FoxJ1(+), Cilia(–)), FoxJ1-negative cilia-positive cells (FoxJ1(–), Cilia(+)) among the total cells in each condition are shown. The number of FoxJ1-negative cilia-positive cells among the total cells was less than 0.5%. 50 ng of BMP2 or BMP4 is supplemented in 1 mL medium in the BMP conditions. n = 3. C. The number of nestin-positive cells among the total cells in each condition are shown. 50 ng of BMP2 or BMP4 is supplemented in 1 mL medium in the BMP conditions. Data are mean ± SEM; n = 3.
Article Snippet: In the second experiment,
Techniques: Cell Culture, Control, Marker, Staining