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Fig. 3. Effect of bone morphogenetic proteins (BMPs) on cell differentiation. The number of multi-ciliated cells per total cells (A), the density of multi-ciliated cells (B), and the density of total cells (C) in different BMPs concentrations and medium with vehicle (control) are shown. The same control data is used for analyses of both <t>BMP2</t> conditions and BMP4 conditions. n = 8 or 9. Data are mean ± SEM. “†” indicates a significant difference between day 0 and the other days. “*” indicates significant difference between days except day 0. *p < 0.05, **p < 0.01, ***, †††p < 0.0001.
Recombinant Human Mouse Rat Bmp2 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 3. Effect of bone morphogenetic proteins (BMPs) on cell differentiation. The number of multi-ciliated cells per total cells (A), the density of multi-ciliated cells (B), and the density of total cells (C) in different BMPs concentrations and medium with vehicle (control) are shown. The same control data is used for analyses of both <t>BMP2</t> conditions and BMP4 conditions. n = 8 or 9. Data are mean ± SEM. “†” indicates a significant difference between day 0 and the other days. “*” indicates significant difference between days except day 0. *p < 0.05, **p < 0.01, ***, †††p < 0.0001.
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Fig. 3. Effect of bone morphogenetic proteins (BMPs) on cell differentiation. The number of multi-ciliated cells per total cells (A), the density of multi-ciliated cells (B), and the density of total cells (C) in different BMPs concentrations and medium with vehicle (control) are shown. The same control data is used for analyses of both <t>BMP2</t> conditions and BMP4 conditions. n = 8 or 9. Data are mean ± SEM. “†” indicates a significant difference between day 0 and the other days. “*” indicates significant difference between days except day 0. *p < 0.05, **p < 0.01, ***, †††p < 0.0001.
Bmp2 Recombinant Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 3. Effect of bone morphogenetic proteins (BMPs) on cell differentiation. The number of multi-ciliated cells per total cells (A), the density of multi-ciliated cells (B), and the density of total cells (C) in different BMPs concentrations and medium with vehicle (control) are shown. The same control data is used for analyses of both <t>BMP2</t> conditions and BMP4 conditions. n = 8 or 9. Data are mean ± SEM. “†” indicates a significant difference between day 0 and the other days. “*” indicates significant difference between days except day 0. *p < 0.05, **p < 0.01, ***, †††p < 0.0001.
Recombinant Bmp2 Protein R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 3. Effect of bone morphogenetic proteins (BMPs) on cell differentiation. The number of multi-ciliated cells per total cells (A), the density of multi-ciliated cells (B), and the density of total cells (C) in different BMPs concentrations and medium with vehicle (control) are shown. The same control data is used for analyses of both <t>BMP2</t> conditions and BMP4 conditions. n = 8 or 9. Data are mean ± SEM. “†” indicates a significant difference between day 0 and the other days. “*” indicates significant difference between days except day 0. *p < 0.05, **p < 0.01, ***, †††p < 0.0001.
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Image Search Results


Journal: Immunity

Article Title: Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche

doi: 10.1016/j.immuni.2019.08.017

Figure Lengend Snippet:

Article Snippet: Recombinant Human/Mouse/Rat BMP-2 Protein , RnD Systems , Cat#355-BM-010.

Techniques: Control, Recombinant, Blocking Assay, Irradiation, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Microarray, Software, Microscopy

Fig. 3. Effect of bone morphogenetic proteins (BMPs) on cell differentiation. The number of multi-ciliated cells per total cells (A), the density of multi-ciliated cells (B), and the density of total cells (C) in different BMPs concentrations and medium with vehicle (control) are shown. The same control data is used for analyses of both BMP2 conditions and BMP4 conditions. n = 8 or 9. Data are mean ± SEM. “†” indicates a significant difference between day 0 and the other days. “*” indicates significant difference between days except day 0. *p < 0.05, **p < 0.01, ***, †††p < 0.0001.

Journal: The Tohoku journal of experimental medicine

Article Title: Bone Morphogenetic Proteins Inhibit Ciliogenesis of Ependymal Cells in Vitro.

doi: 10.1620/tjem.252.199

Figure Lengend Snippet: Fig. 3. Effect of bone morphogenetic proteins (BMPs) on cell differentiation. The number of multi-ciliated cells per total cells (A), the density of multi-ciliated cells (B), and the density of total cells (C) in different BMPs concentrations and medium with vehicle (control) are shown. The same control data is used for analyses of both BMP2 conditions and BMP4 conditions. n = 8 or 9. Data are mean ± SEM. “†” indicates a significant difference between day 0 and the other days. “*” indicates significant difference between days except day 0. *p < 0.05, **p < 0.01, ***, †††p < 0.0001.

Article Snippet: In the second experiment, recombinant human/mouse/ rat BMP2 protein (R&D systems, 355-BM-010) or recombinant mouse BMP4 protein (R&D systems, 5020-BP-010) was added in DMEM without fetal bovine serum to evaluate the effect of BMP proteins on the differentiation of ependymal cells.

Techniques: Cell Differentiation, Control

Fig. 5. Phosphorylation of Smad1/5/8 proteins by BMP treatment in the primary ependymal cells. A. Signals of phosphorylated Smad1/5/8 (pSmad1/5/8) were detected in BMP2 or BMP4-treated cells (BMP2 or BMP4) but not in the control cells (Control). GAPDH was used as an internal control. B. The signal of non-phosphorylated Smad5 was similar in each condition.

Journal: The Tohoku journal of experimental medicine

Article Title: Bone Morphogenetic Proteins Inhibit Ciliogenesis of Ependymal Cells in Vitro.

doi: 10.1620/tjem.252.199

Figure Lengend Snippet: Fig. 5. Phosphorylation of Smad1/5/8 proteins by BMP treatment in the primary ependymal cells. A. Signals of phosphorylated Smad1/5/8 (pSmad1/5/8) were detected in BMP2 or BMP4-treated cells (BMP2 or BMP4) but not in the control cells (Control). GAPDH was used as an internal control. B. The signal of non-phosphorylated Smad5 was similar in each condition.

Article Snippet: In the second experiment, recombinant human/mouse/ rat BMP2 protein (R&D systems, 355-BM-010) or recombinant mouse BMP4 protein (R&D systems, 5020-BP-010) was added in DMEM without fetal bovine serum to evaluate the effect of BMP proteins on the differentiation of ependymal cells.

Techniques: Phospho-proteomics, Control

Fig. 7. FoxJ1 and nestin expressions in the cultured cells. A. Representative image of cells cultured in medium without BMPs (control condition) for 14 days. A cilia marker, β-Tubulin IV, is stained in green, and FoxJ1, a nuclear marker for ciliated cells is stained in magenta. The nuclei are counterstained with DAPI in blue. Arrows indicate FoxJ1-positive cells without multiple cilia, and arrowheads indi- cate FoxJ1-positive cells with multiple cilia. Scale bar: 20 µm. B. The number of FoxJ1-positive cilia-positive cells (FoxJ1(+), Cilia(+)), FoxJ1-positive cilia-negative cells (FoxJ1(+), Cilia(–)), FoxJ1-negative cilia-positive cells (FoxJ1(–), Cilia(+)) among the total cells in each condition are shown. The number of FoxJ1-negative cilia-positive cells among the total cells was less than 0.5%. 50 ng of BMP2 or BMP4 is supplemented in 1 mL medium in the BMP conditions. n = 3. C. The number of nestin-positive cells among the total cells in each condition are shown. 50 ng of BMP2 or BMP4 is supplemented in 1 mL medium in the BMP conditions. Data are mean ± SEM; n = 3.

Journal: The Tohoku journal of experimental medicine

Article Title: Bone Morphogenetic Proteins Inhibit Ciliogenesis of Ependymal Cells in Vitro.

doi: 10.1620/tjem.252.199

Figure Lengend Snippet: Fig. 7. FoxJ1 and nestin expressions in the cultured cells. A. Representative image of cells cultured in medium without BMPs (control condition) for 14 days. A cilia marker, β-Tubulin IV, is stained in green, and FoxJ1, a nuclear marker for ciliated cells is stained in magenta. The nuclei are counterstained with DAPI in blue. Arrows indicate FoxJ1-positive cells without multiple cilia, and arrowheads indi- cate FoxJ1-positive cells with multiple cilia. Scale bar: 20 µm. B. The number of FoxJ1-positive cilia-positive cells (FoxJ1(+), Cilia(+)), FoxJ1-positive cilia-negative cells (FoxJ1(+), Cilia(–)), FoxJ1-negative cilia-positive cells (FoxJ1(–), Cilia(+)) among the total cells in each condition are shown. The number of FoxJ1-negative cilia-positive cells among the total cells was less than 0.5%. 50 ng of BMP2 or BMP4 is supplemented in 1 mL medium in the BMP conditions. n = 3. C. The number of nestin-positive cells among the total cells in each condition are shown. 50 ng of BMP2 or BMP4 is supplemented in 1 mL medium in the BMP conditions. Data are mean ± SEM; n = 3.

Article Snippet: In the second experiment, recombinant human/mouse/ rat BMP2 protein (R&D systems, 355-BM-010) or recombinant mouse BMP4 protein (R&D systems, 5020-BP-010) was added in DMEM without fetal bovine serum to evaluate the effect of BMP proteins on the differentiation of ependymal cells.

Techniques: Cell Culture, Control, Marker, Staining